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Macromolecular X-ray and Computing X-ray Facility

The facility is located at L141 of the main campus, 3333 无码群交, North Chicago, IL 60064. It supports structural biology projects based on X-ray crystallography, single particle cryo electron microscopy and molecular modeling. 无码群交 is a member of SER-CAT () with regular access to synchrotron beamlines 22ID and 22BM at Advanced Photon Source.  CryoEM data is collected at National Facilities including S2C2 and NCCAT ().  Linux computing includes a 15TB-4GPU workstation for the cryoEM data processing and several graphics workstations for the x-ray crystallography.  Multiple software suites are kept current by Structural Biology Grid (). Modeling packages include Molecular Operating Environment () and SCHRODINGER (). The facility have equipment as listed below.

The Core Investigators

INVESTIGATOR NAME

PROJECT

David H. T. Harrison, PhD, Director BMB secondary appointment

Structural Enzymology, Methylglyoxal Synthase, HMG-CoA Synthase, and Hydroxyphenyl pyruvate

Henry Symersky, PhD Facilities Manager

Departmental projects utilizing macromolecular x-ray crystallography

Carl Correll, PhD, BMB

RNA Chaperones

Marc Glucksman, PhD, BMB

Structural neurobiology of processing enzymes

Ronald Kaplan, PhD, BMB and Vice Dean of Research (CMS)

Mitochondrial transport proteins

Min Lu, PhD, BMB

Multidrug and Toxin Extrusion (MATE) transporters

David Mueller, PhD, BMB

Yeast ATP synthase and cystic fibrosis transmembrane conductance regulator

Kyoung Joon Oh, PhD,BMB

Program cell death pathways

Eric Walters, PhD, BMB

HIV-1 protease/non-peptidyl inhibitors

Dmitri Boudko, PhD, PB

 Transporters

Maintenance Information:

  • Confocal mirror 
  • Oxford Cryostream 
  • Rigaku/MSC X-Stream 
  • Rigaku/MSC R-axis IV detector 
  • Rigaku/MSC RU-h2R 
  • Rigaku/MSC RU-h2R 

Useful X-ray DataProcessing guides:

RFUMS X-ray Facility Resources:

  • Rigaku RU-h2R 5.4 kW X-ray generator with copper rotating anode 
  • Osmic confocal “green” fine focusing mirrors 
  • R-axis IV++ imaging plate area detector 
  • CCD video system for crystal mounting 
  • MSC X-Stream 2000 cryocooling system 
  • Bruker AXS SMART 6000 CCD detector with 3-circle goniometer 
  • Oxford cryocooling system 
  • TPP Labtech Mosquito crystallization robot 
  • Korima UV crystal detection and visualization system 
  • Liquid nitrogen 
  • Storage liquid nitrogen dewars 
  • Dry-shipping dewars 
  • Cryotools and crystal handling tools 
  • Six large incubators with temperature control 
  • Temperature-controlled room for crystal growth and manipulations 

RFUMS CryoEM  Facility Resources:

  • Linux 15TB-4GPU workstation.
  • PELCO easiGlowTM Glow Discharge system.
  • EOL JEM-1230, 120kV Transmission Electron Microscope (TEM) equipped with a high-resolution Hamamatsu ORCA-HR CCD Camera (2624 x 2400 pixel) with AMT Imaging Software for negative stained images.  

Building:  BSB
Room:  L.141
Phone:  847-578-8474